Renaturation of transfer ribonucleic acids through site binding of magnesium.
نویسندگان
چکیده
منابع مشابه
Isolation of "renaturable" transfer ribonucleic acids.
A new type of fractionating step involving gel Cltration is introduced for the purification of certain transfer ribonucleic acids (tRNAs). It is based on the increase that occurs in the molecular dimensions of these tRNAs when they are trapped, reversibly, in a denatured state under conditions where the other tRNA components of the mixture are in the native conformation. This step, coupled to c...
متن کاملIsolation of Specific Transfer Ribonucleic Acids
1. HAGELBERG, L., Ber. de&. hem. Ges., 23,1083 (1890). 2. KATJFMANN. H. P.. GINDSBERG. E.. ROTTIG, W.. AND SALCHOW, R., Ber. $eut. chkm. Ges., 70, 2519 (1937). ’ ’ 3. SIEGEL, J. R., AND ROSENBLATT, D. H., J. Am. Chem. Sot., 80, 1753 (1958). 4. GABRIEL, S., Ber. deut. them. Ges., 22, 1139 (1889). 5. FIESER, i. I?., Experiments in organic chemistry, Ed. 2, D. C. Heath and Co., New York, 1941, p. ...
متن کاملProflavine binding to transfer ribonucleic acid, synthetic ribonucleic acids, and deoxyribonucleic acid.
The ability of proflavine to directly inhibit protein synthesis has led to an investigation of complex formation between the drug and ribonucleic acid. Evidence has been obtained from absorption and fluorescence spectra, as well as heat denaturation curves, that proflavine forms a complex with transfer ribonucleic acid which is qualitatively similar to the deoxyribonucleic acid-proflavine compl...
متن کاملProflavine Binding to Transfer Ribonucleic Acid, Synthetic Ribonucleic Acids, and Deoxyribonucleic Acid*
The ability of proflavine to directly inhibit protein synthesis has led to an investigation of complex formation between the drug and ribonucleic acid. Evidence has been obtained from absorption and fluorescence spectra, as well as heat denaturation curves, that proflavine forms a complex with transfer ribonucleic acid which is qualitatively similar to the deoxyribonucleic acid-proflavine compl...
متن کاملThe binding of transfer ribonucleic acids to 5 S and 5.8 S eukaryotic ribosomal ribonucleic acid-protein complexes.
Rat liver 5 S and 5.8 S rRNAs were oxidized with periodate and the 3' termini were coupled to Sepharose 4B through an adipic acid dihydrazide spacer. Ribosomal proteins were passed through the nucleic acid affinity columns to form ribonucleoprotein complexes containing the nucleic acid and the proteins that bind to it (5 S . L6, L7, L19; and 5.8 S . L6, L19, S9, S13). Pure isoaccepting species ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1966
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.55.4.941